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primer extension  (New England Biolabs)
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New England Biolabs primer extension
Primer Extension, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primer extension  (TaKaRa)
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sars cov 2 rdrp primer extension assays  (MedChemExpress)
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MedChemExpress sars cov 2 rdrp primer extension assays
NSC93472 interacts MHV RNA in vitro and in vivo . A , SPR response and titration curves for NSC93472 <t>binding</t> <t>with</t> <t>SARS-CoV-2</t> F593-M RNA. NSC93472 shows binding greater than 1:1 at 5 μM. The dashed line indicates the theoretical max SPR response (R-max) for 1:1 binding event. B , SPR responses of NSC93472 binding with F593-M RNA and S2m RNA, and an ssRNA. C , NSC93472 localization in HEK293 cells. Cells were treated with 1 μM NSC93472 for 1 h and stained with nucleolar stain (ab139475). D , colocalization of NSC93472 and nsp2-GFP in 17Cl-1 cells infected by MHV-nsp2-GFP. 17Cl-1 cells were infected with 0.02 MOI MHV-nsp2-GFP (nsp2 is an MHV RdRp component) for 16 h, and then incubated with 0.2 μM NSC93472 for 8 h. The nsp2-GFP signals that are overlapped and nonoverlapped with NSC93472 in cells are indicated by white and yellow arrows , respectively. HEK293, human embryonic kidney 293 cells; MHV, mouse hepatitis coronavirus; MOI, multiplicity of infection; RdRp, RNA-dependent RNA polymerase; SPR, surface plasmon resonance.
Sars Cov 2 Rdrp Primer Extension Assays, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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forward extension primer  (Thermo Fisher)
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Thermo Fisher forward extension primer
NSC93472 interacts MHV RNA in vitro and in vivo . A , SPR response and titration curves for NSC93472 <t>binding</t> <t>with</t> <t>SARS-CoV-2</t> F593-M RNA. NSC93472 shows binding greater than 1:1 at 5 μM. The dashed line indicates the theoretical max SPR response (R-max) for 1:1 binding event. B , SPR responses of NSC93472 binding with F593-M RNA and S2m RNA, and an ssRNA. C , NSC93472 localization in HEK293 cells. Cells were treated with 1 μM NSC93472 for 1 h and stained with nucleolar stain (ab139475). D , colocalization of NSC93472 and nsp2-GFP in 17Cl-1 cells infected by MHV-nsp2-GFP. 17Cl-1 cells were infected with 0.02 MOI MHV-nsp2-GFP (nsp2 is an MHV RdRp component) for 16 h, and then incubated with 0.2 μM NSC93472 for 8 h. The nsp2-GFP signals that are overlapped and nonoverlapped with NSC93472 in cells are indicated by white and yellow arrows , respectively. HEK293, human embryonic kidney 293 cells; MHV, mouse hepatitis coronavirus; MOI, multiplicity of infection; RdRp, RNA-dependent RNA polymerase; SPR, surface plasmon resonance.
Forward Extension Primer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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single base extension primers  (Sequenom)
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Sequenom single base extension primers
NSC93472 interacts MHV RNA in vitro and in vivo . A , SPR response and titration curves for NSC93472 <t>binding</t> <t>with</t> <t>SARS-CoV-2</t> F593-M RNA. NSC93472 shows binding greater than 1:1 at 5 μM. The dashed line indicates the theoretical max SPR response (R-max) for 1:1 binding event. B , SPR responses of NSC93472 binding with F593-M RNA and S2m RNA, and an ssRNA. C , NSC93472 localization in HEK293 cells. Cells were treated with 1 μM NSC93472 for 1 h and stained with nucleolar stain (ab139475). D , colocalization of NSC93472 and nsp2-GFP in 17Cl-1 cells infected by MHV-nsp2-GFP. 17Cl-1 cells were infected with 0.02 MOI MHV-nsp2-GFP (nsp2 is an MHV RdRp component) for 16 h, and then incubated with 0.2 μM NSC93472 for 8 h. The nsp2-GFP signals that are overlapped and nonoverlapped with NSC93472 in cells are indicated by white and yellow arrows , respectively. HEK293, human embryonic kidney 293 cells; MHV, mouse hepatitis coronavirus; MOI, multiplicity of infection; RdRp, RNA-dependent RNA polymerase; SPR, surface plasmon resonance.
Single Base Extension Primers, supplied by Sequenom, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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m13ke insert extension primer  (New England Biolabs)
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New England Biolabs m13ke insert extension primer
NSC93472 interacts MHV RNA in vitro and in vivo . A , SPR response and titration curves for NSC93472 <t>binding</t> <t>with</t> <t>SARS-CoV-2</t> F593-M RNA. NSC93472 shows binding greater than 1:1 at 5 μM. The dashed line indicates the theoretical max SPR response (R-max) for 1:1 binding event. B , SPR responses of NSC93472 binding with F593-M RNA and S2m RNA, and an ssRNA. C , NSC93472 localization in HEK293 cells. Cells were treated with 1 μM NSC93472 for 1 h and stained with nucleolar stain (ab139475). D , colocalization of NSC93472 and nsp2-GFP in 17Cl-1 cells infected by MHV-nsp2-GFP. 17Cl-1 cells were infected with 0.02 MOI MHV-nsp2-GFP (nsp2 is an MHV RdRp component) for 16 h, and then incubated with 0.2 μM NSC93472 for 8 h. The nsp2-GFP signals that are overlapped and nonoverlapped with NSC93472 in cells are indicated by white and yellow arrows , respectively. HEK293, human embryonic kidney 293 cells; MHV, mouse hepatitis coronavirus; MOI, multiplicity of infection; RdRp, RNA-dependent RNA polymerase; SPR, surface plasmon resonance.
M13ke Insert Extension Primer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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extension primers  (Sequenom)
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Sequenom extension primers
NSC93472 interacts MHV RNA in vitro and in vivo . A , SPR response and titration curves for NSC93472 <t>binding</t> <t>with</t> <t>SARS-CoV-2</t> F593-M RNA. NSC93472 shows binding greater than 1:1 at 5 μM. The dashed line indicates the theoretical max SPR response (R-max) for 1:1 binding event. B , SPR responses of NSC93472 binding with F593-M RNA and S2m RNA, and an ssRNA. C , NSC93472 localization in HEK293 cells. Cells were treated with 1 μM NSC93472 for 1 h and stained with nucleolar stain (ab139475). D , colocalization of NSC93472 and nsp2-GFP in 17Cl-1 cells infected by MHV-nsp2-GFP. 17Cl-1 cells were infected with 0.02 MOI MHV-nsp2-GFP (nsp2 is an MHV RdRp component) for 16 h, and then incubated with 0.2 μM NSC93472 for 8 h. The nsp2-GFP signals that are overlapped and nonoverlapped with NSC93472 in cells are indicated by white and yellow arrows , respectively. HEK293, human embryonic kidney 293 cells; MHV, mouse hepatitis coronavirus; MOI, multiplicity of infection; RdRp, RNA-dependent RNA polymerase; SPR, surface plasmon resonance.
Extension Primers, supplied by Sequenom, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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reverse extension primer  (Thermo Fisher)
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Thermo Fisher reverse extension primer
NSC93472 interacts MHV RNA in vitro and in vivo . A , SPR response and titration curves for NSC93472 <t>binding</t> <t>with</t> <t>SARS-CoV-2</t> F593-M RNA. NSC93472 shows binding greater than 1:1 at 5 μM. The dashed line indicates the theoretical max SPR response (R-max) for 1:1 binding event. B , SPR responses of NSC93472 binding with F593-M RNA and S2m RNA, and an ssRNA. C , NSC93472 localization in HEK293 cells. Cells were treated with 1 μM NSC93472 for 1 h and stained with nucleolar stain (ab139475). D , colocalization of NSC93472 and nsp2-GFP in 17Cl-1 cells infected by MHV-nsp2-GFP. 17Cl-1 cells were infected with 0.02 MOI MHV-nsp2-GFP (nsp2 is an MHV RdRp component) for 16 h, and then incubated with 0.2 μM NSC93472 for 8 h. The nsp2-GFP signals that are overlapped and nonoverlapped with NSC93472 in cells are indicated by white and yellow arrows , respectively. HEK293, human embryonic kidney 293 cells; MHV, mouse hepatitis coronavirus; MOI, multiplicity of infection; RdRp, RNA-dependent RNA polymerase; SPR, surface plasmon resonance.
Reverse Extension Primer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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NSC93472 interacts MHV RNA in vitro and in vivo . A , SPR response and titration curves for NSC93472 binding with SARS-CoV-2 F593-M RNA. NSC93472 shows binding greater than 1:1 at 5 μM. The dashed line indicates the theoretical max SPR response (R-max) for 1:1 binding event. B , SPR responses of NSC93472 binding with F593-M RNA and S2m RNA, and an ssRNA. C , NSC93472 localization in HEK293 cells. Cells were treated with 1 μM NSC93472 for 1 h and stained with nucleolar stain (ab139475). D , colocalization of NSC93472 and nsp2-GFP in 17Cl-1 cells infected by MHV-nsp2-GFP. 17Cl-1 cells were infected with 0.02 MOI MHV-nsp2-GFP (nsp2 is an MHV RdRp component) for 16 h, and then incubated with 0.2 μM NSC93472 for 8 h. The nsp2-GFP signals that are overlapped and nonoverlapped with NSC93472 in cells are indicated by white and yellow arrows , respectively. HEK293, human embryonic kidney 293 cells; MHV, mouse hepatitis coronavirus; MOI, multiplicity of infection; RdRp, RNA-dependent RNA polymerase; SPR, surface plasmon resonance.

Journal: The Journal of Biological Chemistry

Article Title: Discovery of thiacyanine dyes as a new class of potent coronavirus inhibitors that suppress viral RNA synthesis

doi: 10.1016/j.jbc.2025.110547

Figure Lengend Snippet: NSC93472 interacts MHV RNA in vitro and in vivo . A , SPR response and titration curves for NSC93472 binding with SARS-CoV-2 F593-M RNA. NSC93472 shows binding greater than 1:1 at 5 μM. The dashed line indicates the theoretical max SPR response (R-max) for 1:1 binding event. B , SPR responses of NSC93472 binding with F593-M RNA and S2m RNA, and an ssRNA. C , NSC93472 localization in HEK293 cells. Cells were treated with 1 μM NSC93472 for 1 h and stained with nucleolar stain (ab139475). D , colocalization of NSC93472 and nsp2-GFP in 17Cl-1 cells infected by MHV-nsp2-GFP. 17Cl-1 cells were infected with 0.02 MOI MHV-nsp2-GFP (nsp2 is an MHV RdRp component) for 16 h, and then incubated with 0.2 μM NSC93472 for 8 h. The nsp2-GFP signals that are overlapped and nonoverlapped with NSC93472 in cells are indicated by white and yellow arrows , respectively. HEK293, human embryonic kidney 293 cells; MHV, mouse hepatitis coronavirus; MOI, multiplicity of infection; RdRp, RNA-dependent RNA polymerase; SPR, surface plasmon resonance.

Article Snippet: For SARS-CoV-2 RdRp primer extension assays, Nsp12 (400–700 nM), Nsp7 (7200 nM), and Nsp8 (7200 nM) were incubated with the indicated cyanine dye, PSI-7409 tetrasodium (Medchem Express), or the solvent carrier DMSO (concentration indicated in figure legend) and the 78-nt F593-M template RNA substrate annealed to either a 15-nt or 31-nt RNA primer (80 nM).

Techniques: In Vitro, In Vivo, Titration, Binding Assay, Staining, Infection, Incubation, SPR Assay